Question 1

Which of the following describes the major difference between the gene expression strategies between different members of the parvovirus family?

Accepted Answer

A)  Some use alternative mRNA splicing and some do not.
B)  They use different numbers of transcriptional promoters.
C)  Some use alternative start codons and some do not.
D)  Some use RNA editing and some do not.E) A) and B)
F) C) and D)

Question 2

Parvoviruses can only productively infect cells that are NOT going through the cell cycle.

Accepted Answer

A) True 
 B)False

Question 3

The absence of the minor capsid proteins in parvovirus virions does not reduce infectivity.

Accepted Answer

A) True 
 B)False

Question 4

What major function does coinfection with an adenovirus provide to adeno-associated virus,a member of the dependovirus group of parvoviruses?

Accepted Answer

A)  It helps the parvovirus package new virions.
B)  It helps the parvovirus enter the nucleus.
C)  It induces the host cell to enter S phase.
D)  It provides a DNA polymerase for genome replication.
E)  It provides an RNA polymerase for gene expression.F) None of the above
G) All of the above

Question 5

Eukaryotic cellular chromosomes use the same mechanism as the parvoviruses to maintain the ends of their linear DNA.

Accepted Answer

A) True 
 B)False

Question 6

Which of the following describes the first step in the replication cycle of the autonomous parvoviruses?

Accepted Answer

A)  Translation of the incoming genome into proteins.
B)  Transcription of the incoming genome into early mRNAs.
C)  Synthesis of double-stranded DNA from the incoming genome.
D)  Alterative splicing of the incoming genome.
E)  Integration of the incoming genome into the host chromosome.F) A) and E)
G) D) and E)

Question 7

When researchers first isolated the DNA genomes from the virions of the adeno-assocaited virus,a member of the dependovirus group of parvoviruses,they thought they were double-stranded.Explain why and how they were able to determine the correct genome structure.

Accepted Answer

Adeno-associated virus packages either the positive-sense strand of DNA or the negative-sense strand of DNA into each virion.While a single virion will only have a single-molecule of single-stranded DNA in each particle,a collection of particles will have nearly an equal number of both positive and negative sense strands.When the DNA was released from the particles it reannealed to its complementary strand and the genome appeared to be double-stranded.This was puzzling because the AAV particles are not large enough to hold a double-stranded DNA molecule.When the researchers used conditions,such as high temperature or high pH,where the two strands could not reanneal,they observed the result of single-stranded DNA molecules.

Question 8

Which of the following steps has been shown to be blocked in cells that are nonpermissive for parvoviruses?

Accepted Answer

A)  Binding of the virion to the correct receptor.
B)  Uncoating of the virion.
C)  Transcription of viral mRNAs.
D)  Synthesis of viral proteins.
E)  Replication of the viral genome.F) B) and E)
G) C) and E) B

Question 9

Which of the following describes the structures found at the ends of the parvovirus genomes?

Accepted Answer

A)  Self-complementary hairpin structures.
B)  5' cap structures and 3' poly(
A)  tails.
C)  5' triphosphate.
D)  Covalently attached proteins.
E)  The genome is circular so it has no ends.F) B) and E)
G) A) and E)

Question 10

Explain why the ends of DNA can not be completely replicated and how the parvoviruses use the self-complementary ends to solve this problem.

Accepted Answer

All DNA polymerases require a pre-existi...

Question 11

The problem with replicating the ends of linear DNA is due to which of the following?

Accepted Answer

A)  That DNA polymerase does not require a pre-existing primer.
B)  The removal of the primer from the 3' end of the new DNA strand.
C)  The removal of the primer from the 5' end of the new DNA strand.
D)  That the primer is composed of RNA rather than DNA.
E)  That DNA polymerases can not synthesize DNA in the 5' to 3' direction.F) B) and C)
G) A) and C)

Question 12

How do parvoviruses solve the problem of replicating the ends of linear DNA molecules?

Accepted Answer

A)  They do not have a double-stranded DNA genome.
B)  They circularize their genome so that there are no ends.
C)  They use a covalently bound protein as a primer.
D)  They have self-complementary ends that serve as primers.
E)  They encode a special DNA polymerase that can replicate ends of DNA.F) B) and D)
G) C) and D)

Question 13

Which of the following describes the fate of the AAV parvovirus genome in the absence of a helper virus?

Accepted Answer

A)  It is degraded.
B)  It integrates into a nonspecific location on a human chromosome.
C)  It integrates into a specific location on chromosome 19.
D)  It is replicated,just more slowly in the absence of the helper virus.
E)  It is maintained as an episome in the nucleus of the infected cell.F) C) and D)
G) A) and D)

Question 14

Which of the following does NOT explain why parvoviruses replicate better in tumor cells than in normal cells?

Accepted Answer

A)  Tumor cells have a reduced interferon response than normal cells.
B)  Tumor cells bind to more parvovirus virions than normal cells.
C)  Tumor cells have reduced DNA damage response than normal cells.
D)  Tumor cells go through the cell cycle more quickly than normal cells.
E)  None of the above.F) C) and D)
G) None of the above

Question 15

Which of the following diseases is NOT caused by a parvovirus?

Accepted Answer

A)  Panleukopenia
B)  Aplastic crisis in anemia patients
C)  Erythema infectiosum
D)  Birth defects
E)  Neurological problems.F) D) and E)
G) A) and C)

Question 16

Which of the following is NOT a function of the NS1 protein from minute virus of mice,an autonomous parvovirus?

Accepted Answer

A)  Stimulates transcription from its own p4 promoter.
B)  Stimulates transcription from the p38 promoter.
C)  Unwinds DNA hairpins during genome replication.
D)  Nicks viral DNA during genome replication.
E)  Regulates splicing of the viral mRNAs.F) A) and B)
G) A) and E)

Question 17

Which of the following distinguishes the parvoviruses from adenoviruses and polyomaviruses?

Accepted Answer

A)  They have naked capsids.
B)  They replicate in the nucleus.
C)  They require the cellular DNA polymerase.
D)  They cannot induce the cell to enter S phase.
E)  They have a DNA genome.F) B) and D)
G) A) and D)

Question 18

The oncotropism exhibited by parvoviruses means that they replicate more efficiently in tumor cells.

Accepted Answer

A) True 
 B)False  True

Question 19

Which of the following describes the type of nucleic acid used in the genomes of parvoviruses?

Accepted Answer

A)  Single-stranded DNA.
B)  Double-stranded DNA.
C)  Positive-sense single-stranded RNA.
D)  Negative-sense single-stranded RNA.
E)  Double-stranded RNA.F) A) and C)
G) C) and D)

Question 20

Which of the following viral proteins is responsible for the integration of the genome of the parvovirus adeno-associate virus into the host chromosome?

Accepted Answer

A)  Rep40
B)  Rep78
C)  VP1
D)  VP2
E)  integraseF) None of the above
G) A) and C)

Exam 20: Parvoviruses

Which of the following describes the major difference between the gene expression strategies between different members of the parvovirus family?

Correct Answer
verified

Parvoviruses can only productively infect cells that are NOT going through the cell cycle.

Correct Answer
verified

The absence of the minor capsid proteins in parvovirus virions does not reduce infectivity.

Correct Answer
verified

What major function does coinfection with an adenovirus provide to adeno-associated virus,a member of the dependovirus group of parvoviruses?

Correct Answer
verified

Eukaryotic cellular chromosomes use the same mechanism as the parvoviruses to maintain the ends of their linear DNA.

Correct Answer
verified

Which of the following describes the first step in the replication cycle of the autonomous parvoviruses?

Correct Answer
verified

When researchers first isolated the DNA genomes from the virions of the adeno-assocaited virus,a member of the dependovirus group of parvoviruses,they thought they were double-stranded.Explain why and how they were able to determine the correct genome structure.

Which of the following steps has been shown to be blocked in cells that are nonpermissive for parvoviruses?

Correct Answer
verified
B

Which of the following describes the structures found at the ends of the parvovirus genomes?

Correct Answer
verified

Explain why the ends of DNA can not be completely replicated and how the parvoviruses use the self-complementary ends to solve this problem.

Correct Answer
verified
All DNA polymerases require a pre-existi...
View Answer

The problem with replicating the ends of linear DNA is due to which of the following?

Correct Answer
verified

How do parvoviruses solve the problem of replicating the ends of linear DNA molecules?

Correct Answer
verified

Which of the following describes the fate of the AAV parvovirus genome in the absence of a helper virus?

Correct Answer
verified

Which of the following does NOT explain why parvoviruses replicate better in tumor cells than in normal cells?

Correct Answer
verified

Which of the following diseases is NOT caused by a parvovirus?

Correct Answer
verified

Which of the following is NOT a function of the NS1 protein from minute virus of mice,an autonomous parvovirus?

Correct Answer
verified

Which of the following distinguishes the parvoviruses from adenoviruses and polyomaviruses?

Correct Answer
verified

The oncotropism exhibited by parvoviruses means that they replicate more efficiently in tumor cells.

Correct Answer
verified
True

Which of the following describes the type of nucleic acid used in the genomes of parvoviruses?

Correct Answer
verified

Which of the following viral proteins is responsible for the integration of the genome of the parvovirus adeno-associate virus into the host chromosome?

Correct Answer
verified

Exam 20: Parvoviruses

Which of the following describes the major difference between the gene expression strategies between different members of the parvovirus family?

Correct AnswerverifiedShow Answer

Parvoviruses can only productively infect cells that are NOT going through the cell cycle.

Correct AnswerverifiedShow Answer

The absence of the minor capsid proteins in parvovirus virions does not reduce infectivity.

Correct AnswerverifiedShow Answer

What major function does coinfection with an adenovirus provide to adeno-associated virus,a member of the dependovirus group of parvoviruses?

Correct AnswerverifiedShow Answer

Eukaryotic cellular chromosomes use the same mechanism as the parvoviruses to maintain the ends of their linear DNA.

Correct AnswerverifiedShow Answer

Which of the following describes the first step in the replication cycle of the autonomous parvoviruses?

Correct AnswerverifiedShow Answer

When researchers first isolated the DNA genomes from the virions of the adeno-assocaited virus,a member of the dependovirus group of parvoviruses,they thought they were double-stranded.Explain why and how they were able to determine the correct genome structure.

Which of the following steps has been shown to be blocked in cells that are nonpermissive for parvoviruses?

Correct AnswerverifiedB

Which of the following describes the structures found at the ends of the parvovirus genomes?

Correct AnswerverifiedShow Answer

Explain why the ends of DNA can not be completely replicated and how the parvoviruses use the self-complementary ends to solve this problem.

Correct AnswerverifiedAll DNA polymerases require a pre-existi...View AnswerShow Answer

The problem with replicating the ends of linear DNA is due to which of the following?

Correct AnswerverifiedShow Answer

How do parvoviruses solve the problem of replicating the ends of linear DNA molecules?

Correct AnswerverifiedShow Answer

Which of the following describes the fate of the AAV parvovirus genome in the absence of a helper virus?

Correct AnswerverifiedShow Answer

Which of the following does NOT explain why parvoviruses replicate better in tumor cells than in normal cells?

Correct AnswerverifiedShow Answer

Which of the following diseases is NOT caused by a parvovirus?

Correct AnswerverifiedShow Answer

Which of the following is NOT a function of the NS1 protein from minute virus of mice,an autonomous parvovirus?

Correct AnswerverifiedShow Answer

Which of the following distinguishes the parvoviruses from adenoviruses and polyomaviruses?

Correct AnswerverifiedShow Answer

The oncotropism exhibited by parvoviruses means that they replicate more efficiently in tumor cells.

Correct AnswerverifiedTrue

Which of the following describes the type of nucleic acid used in the genomes of parvoviruses?

Correct AnswerverifiedShow Answer

Which of the following viral proteins is responsible for the integration of the genome of the parvovirus adeno-associate virus into the host chromosome?

Correct AnswerverifiedShow Answer

Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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B

Correct Answer
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Correct Answer
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All DNA polymerases require a pre-existi...
View Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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True

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Correct Answer
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